Biography
Biography: Ana Maria Gregio
Abstract
Hyposalivation, xerostomia and alterations in the saliva composition are important side effects related to the use of psychotropic, including the anxiolytics and antidepressants. The mechanism in which these side effects take place has still not been completely clarified; however, histomorphometric and gravimetric studies have been contributing to a better understanding of the cytotoxic effects of anxiolytics drugs on the salivary glands. This study histologically analyzed the parotid glands of Wistar rats that received two benzodiazepines (BZDs) (Lorazepam and Midazolan), associated or not with Pilocarpine (PILO), in order to quantify the number of nuclei of acinar cells (N). Ninety male Wistar rats were allocated to nine groups. Control groups received a saline for 30 days (C30) or 60 days (C60), and (PILO) for 60 days. Experimental groups received lorazepam (L30) and midazolam (M30) for 30 days, lorazepam (LS60) and midazolam (MS60) associated with saline for 60 days, and lorazepam (LP60) and midazolam (MP60) associated with PILO for 60 days. ANOVA and Games-Howell tests were used for statisitical analysis. The L30 and M30 groups presented less N than did the S30 group (p<0.05). The LS60, MS60, and LP60 groups presented less N than did the S60 and P60 groups (p<0.05). No differences could be observed between the MP60 and S60 groups. The administration of Midazolam and Lorazepam reduced acini, which may well have collaborated in the reduction of salivary flow previously verified. The association of Midazolam with Pilocarpine led to the reestablishment of acinar cells, which may have favored the restoration of the salivary flow rate.