5^th American Dental Congress

Biography

Biography: Hossam Elmalahy

Abstract

Purpose: The purpose of this study is to assess the effect of 5-fluorouracil nanoparticles, curcumin naoparticles and combination of both nanoparticles on the expression of the apoptotic marker (caspase 3) in squamous cell carcinoma cell line. Material and methods: Human laryngeal squamous cell carcinoma cell line (Hep-2) was used in this study. 5-fluorouracil nanopartciles and curcumin nanoparticles were prepared and applied in different concentrations for 24 and 48 hours on the used cell line. The yellow tetrazolium salt (MTT) assay was used for evaluation of cytotoxicity of prepared nanoparticles. Real time polymerase chain reaction (RT-PCR) was used for the assessment of caspase-3 expression in the treated cell line. Results: MTT assay revelaed that 5-fluorouracil nanoparticels, curcumin nanoparticels, and combination of both nanoparticles showed a concentration dependant cytotoxicity on Hep-2 cell line. The RT-PCR assessment of caspase-3 expression revealed that there was a concentration dependent increase in caspase-3 expression in Hep-2 cell line treated with different concenrations of 5-fluorouracil nanoparticles, curcumin nanopraticles and combination of both nanoparticles. Conclusion: Curcumin nanoparticles could be more acvtive in inducing apoptosis in short term assays than long term assays due to differential cellular uptake. 5-fluorouracil nanoparticles induced apoptosis could be better detected by RT-PCR rather than by MTT.