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Jothi Varghese

Jothi Varghese

Manipal University, India

Title: A comparative evaluation of three different root conditioning agents on the surface characteristics of cemental surface- SEM study

Biography

Biography: Jothi Varghese

Abstract

The revival of the lost periodontium using therapeutic measures have always been the unceasing objective since decades. Root bio modification agents have been used as an adjunct to periodontal regenerative therapy targeting to demineralize the root surface, thereby providing a matrix which supports periodontal regeneration. Recently, maleic acid has been used as a smear layer removal agent in the field of Endodontics. Hence, the aim of the present study was to evaluate the use of maleic acid as a root bio modification agent and to compare it with three other root conditioning agents for its effectiveness. Method: Twenty extracted single rooted human teeth were selected. Each tooth was decoronated and the apical portion of the root was cut, so as to preserve the mid root portion. Th en, the specimens were randomly assigned to four groups of 10 fragments each according to the demineralizing agents used. Group1 (Citric acid) – application of 50% citric acid, pH 1 for 3 minutes. Group 2 (EDTA) – application of 24% EDTA, pH 7, for 3 minutes. Group 3 (Maleic acid) – application of 7% maleic acid, pH 1.3 for 3 minutes. Group 4 (Control) – application of saline for 3 minutes. Scanning electron microscopy was used to check the presence of residual smear layer and the patency of the dentinal tubules along with fi broblast cell attachment on the cemental surface aft er treatment with the root conditioning agents . Th e results of this study demonstrated that at the 3 min time interval, Maleic acid group, removed 70% of smear layer compared to Citric acid ( 50%) and EDTA ( 30%). Also, all the three experimental groups showed signifi cant fi broblast cell attachment compared to control group. Hence, this study provides suitable information regarding the use of Maleic acid as an denovoagent for root biomodification.