N Vasudev Ballal
Manipal University, India
Title: Evaluation of antibiofi lm activity and smear layer removal effi cacy of a novelendodontic irrigant
Biography
Biography: N Vasudev Ballal
Abstract
Aim of this In vitro study was to evaluateantibiofilm activity and smear layer removal efficacyof a novel endodontic irrigant (NEI). Root canals of fift y single rooted teeth were enlarged with protaper files to size F3. Irrigation was performed with 2.5% NaOCl and 17% EDTA for one minute.All the samples were then coated with nail varnish and sterilized. 5μl of E. faecalis inoculum was placed in the root canals and incubated at 350C for 3 weeks.All the samples were then divided into four groups (n=10). Group 1→ 5ml of 2.5% NaOCl for 5 min; Group 2→ 5 ml of 2% chlorhexidinegluconate (CHX) for 5 min; Group 3→ 25μl of NEIfor 5 min; Group 4→ 5ml of distilled water for 5 min. Th e samples were then longitudinally split and dentin shavings was collected and colony forming units was determined. For smear layer assessment, 30 single rooted teeth were instrumented as mentioned above. Aft er each instrument change, 5 ml of 2.5% NaOCl was used for irrigation. All samples were then divided into 3 groups: Group 1→ 17% EDTA; Group 2→NEI; Group 3→ saline. 5ml of all the irrigantswas used for 1 min. All the specimens were then subjected to SEM analysis. Results demonstrated that, there was no significant diff erence between CHX, NaOCl and NEI related to their antimicrobial eff ect. When smear layer removal efficacy was evaluated, there was no difference between 17% EDTA and NEI in coronal and middle third. However, in apical third 17% EDTA was better.
