Dental & Oral Health

Aim: The aim of this study was to investigate the effect of different double antibiotic paste (DAP) concentrations on viability and the gene expression of collagen type I in dental pulp stromal cells (DPSCs) cultured in presence or absence of TGF ß 1.

 

Methods: DPSCs between passage 6-8 were cultured in-vitro in the presence or absence of TGF β1 growth factor and the presence or absence of two different concentrations of DAP (1.5μg and 25μg) for 3 and 7 days. Cell viability was assessed under the different culture conditions as well as in the basal negative controls at both time points using LDS assay. The gene expression of collagen type 1 under the different culture conditions was investigated using qPCR; the gene expression of all groups was normalized to the expression in DPSCs cultured under basal culture negative control. Statistical analysis was carried out using one-way analysis of variance (ANOVA) and Bonferroni's multiple comparison tests.

 

Results: DPSCs cultured in TGF β1 without antibiotics showed the highest percentage of cell viability after three days. At seven days, the highest percentage of cell viability was in the group cultured with 1.5µg DAP. The 25µg and the 1.5µg DAP groups showed the lowest expression of collagen type I at both time points compared to their culture conditions. The highest expression of collagen type I were noticed at seven days in the 1.5 DAP+TGF β1 group.

 

Conclusion: In this study we have proven that DAP at higher concentration is toxic to DPSCs and hinders their ability to synthesize collagen type I whereas, the lower DAP concentrations were less destructive to DPSCs viability and to collagen type I gene expression. However, lower antibiotic concentrations are more likely to develop microbial resistance.