Masahito Shoumura
Matsumoto Dental University, Japan
Title: Possible role of Notch signaling in the cells from migration of the bone marrow mesenchymal tissues experimetally induced periodontal imflammatrory lesions using GFP BMT-model mice
Biography
Biography: Masahito Shoumura
Abstract
Objective: In our previous examination, the experimentally induced periodontal polyp in mice was examined the cytological dynamics of the lesion by immunohistochemistry using Green Fluorescence Protein bone marrow-transplanted model mice. Our data indicated that the cells in granulation tissue are mainly from migration of undifferentiated mesenchymal cells of the bone marrow, and differentiate into the tissue-specified cells.
Materials and Methods: In the present examination using the same methods using GFP-BMT model mice, the crown of maxillary left first molar of the mouce using ½ round bur to create a perforation of floor of the dental pulp. The regions were examined by histopathology and immunohistochemistry.
Results: Histopathological examination revealed the results suggest that fibroblasts, periodontal ligament fibroblasts and blood vessels in granulation tissue were derived from transplanted-bone marrow cells. Thus, essential growth of granulation tissue in periodontal polyp was caused by the migration of undifferentiated mesenchymal cells derived from bone marrow, which differentiated into fibroblasts and later on differentiated into other cells in response to injury.　The fibroblasts with some round cells and blood vessels were proliferated in the granulation tissue, experimental ranges from at 2 weeks to 6 months. Immunohistochemical staining of Notch1 revealed that the protein was expressed in almost spindle-shaped cells. The result suggests that the periodontal ligament fibroblasts in granulation tissue were expressed Notch1 protein.
Conclusion: Further examination is needed. However, the data strongly suggests that the cell differentiation within the periodontal polyp was controlled by Notch signaling.